Antonio Felix Da Costa put in a late flying lap to go fastest in practice ahead of the second New York E-Prix.
A number of drivers spent time at the top of the order during the session, but it was the Techeetah driver who had the edge when the chequered flag fell.
Despite heavy clouds and a significant chance of rain, the session remained dry throughout.
Robin Frijns set the pace early on, but would soon fall down the order as power modes were increased.
Rene Rast, Jean-Eric Vergne, and Mitch Evans all briefly topped the order.
Late in the session, Pascal Wehrlein went quickest with a 1m08.643s. He wouldn’t stay there for long, as a late flurry of laps caused him to drop down to P7.
In one of the final laps of the session, Da Costa set a 1m08.472s lap to go quickest, 0.049s ahead of Evans.
After a strong performance yesterday, the two Virgin drivers were once again at the front of the order. Nick Cassidy took P3, less than a tenth of a second of the pace set by Da Costa, with Frijns a further 0.032s back.
Rast made it three Audi powered cars in the top five, while Norman Nato was the quickest Mercedes powered driver in P6.
Alex Lynn, Lucas Di Grassi, and Edoardo Mortra completed the top 10.